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The extension of DNA confined to nanochannels has been studied intensively and in detail. However, quantitative comparisons between experiments and model calculations are difficult because most theoretical predictions involve undetermined prefactors, and because the model parameters (contour length, Kuhn length, effective width) are difficult to compute reliably, leading to substantial uncertainti
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This study evaluates a novel method for extracting roots from soil samples and applies it to estimate standing crop root mass (+/- confidence intervals) in an eastern Amazon rainforest. Roots were manually extracted from soil cores over a period of 40 min, which was split into 10 min time intervals. The pattern of cumulative extraction over time was used to predict root extraction beyond 40 min. A
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In this work, we featured an expression system that enables the production of sufficient quantities (~mg) of low molecular weight membrane protein of photosystem II, PsbH protein, for solid-state NMR as well as other biophysical studies. PsbH gene from cyanobacterium Synechocystis sp. PCC 6803 was cloned into a plasmid expression vector, which allowed expression of the PsbH protein as a glutathion
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To determine whether the Joint European Societies guidelines on cardiovascular prevention are being followed in everyday clinical practice of secondary prevention and to describe the lifestyle, risk factor and therapeutic management of coronary patients across Europe.
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Today, in vitro (Latin: in glass) meat researchers strive to overhaul meat production technologies by producing meat outside animal bodies, primarily by culturing cells. In the process, meat should become healthier, more environmentally friendly and kinder to animals. In this article, I scrutinize (and problematize) this promissory discourse by examining the world that proponents envision alongsidToday, in vitro (Latin: in glass) meat researchers strive to overhaul meat production technologies by producing meat outside animal bodies, primarily by culturing cells. In the process, meat should become healthier, more environmentally friendly and kinder to animals. In this article, I scrutinize (and problematize) this promissory discourse by examining the world that proponents envision alongsid
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Colombia has been divided by armed conflict for over half a century. While still confronting multiple forms of violence, since the beginning of the peace talks in 2012 public attention in Colombia has shifted to social reconciliation. In June 2014, Colombians re-elected Juan Manuel Santos as president, his campaign having made peace the centre of attention. The peace negotiations in Havana have be
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This paper describes a new protein chip method for detection of single-base mismatches and unpaired bases of DNA, using a genetic fusion molecular system Trx-His6-Linker peptide-Strep-tagII-Linker peptide-MutS (THLSLM). The THLSLM coding sequence was constructed by attaching Strep-tag II and mutS gene to pET32a (+) sequentially with insertion of a linker peptide coding sequence before and behind S
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This article is focused on gender mainstreaming at Swedish universities in the period from 2016–2019. Our research questions are: (a) In what form was gender mainstreaming introduced and did the form itself affect scholar’s academic rights? (b) Was the process in question compatible with international standards of institutional autonomy? (c) What effect did gender mainstreaming have on scholars’ a
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The article challenges the theoretical “sectoral model” of civil society through a historical case study and offers an alternative actor-centered approach inspired by American pragmatism. First, three separate strands of research are identified that each conceptualize civil society as a sector with institutional independence, a single normative logic, and fixed roles. Building on archival material
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While scholars agree on the reasons behind the current proliferation of urban, small-scale, pre-organised events, the implication of these events for public life is more controversial, and involves polarised debates between enthusiasts and critics. This paper develops an international comparison between one city district in Milan (Italy) and one in Lund (Sweden), in order to explore how the variet
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The Twentieth Century in European Memory investigates contested and divisive memories of conflicts, world wars, dictatorship, genocide and mass killing. Focusing on the questions of transculturality and reception, the book looks at the ways in which such memories are being shared, debated and received by museum workers, artists, politicians and general audiences. Due to amplified mobility and comm
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Thrombospondin 4 (TSP-4) expression is induced in the heart and vasculature under pathological conditions, including myocardial infarction, myocardial pressure overload, and hypertension. TSP-4 is linked to remodelling processes, where it may affect extracellular matrix protein organization. In previous work, we studied the role of TSP-4 in small arteries during hypertension using Ang II-treated T
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Identifying the environmental factors that shape intraspecific genetic and phenotypic diversity of species can provide insights into the processes that generate and maintain divergence in highly diverse biomes such as the savannas of the Neotropics. Here, we sampled Qualea grandiflora, the most widely distributed tree species in the Cerrado, a large Neotropical savanna. We analyzed genetic variati
