Effects of prothrombin on the individual activated protein C-mediated cleavages of coagulation factor Va.
The factor Va (FVa) inactivation by activated protein C (APC), mediated by cleavages at Arg306 and Arg506 in FVa, is inhibited by both factor Xa (FXa) and prothrombin. While FXa is known to specifically inhibit the Arg506 cleavage, the effect of prothrombin has not been confined to one cleavage site. We used recombinant FV variants, FV:506Q/679Q and FV:306Q/679Q, to investigate the effect of proth
