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Effects of Carbon Dioxide on Neurotoxin Gene Expression in Non-proteolytic Clostridium botulinum type E.

Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on growth of, and neurotoxin production by, non-proteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botulin

Urine D-arabinitol/L-arabinitol ratio in diagnosing Candida infection in patients with haematological malignancy and HIV infection

Adult patients with hematologic malignancies along with HIV infected patients were prospectively studied to determine the performance of urine D-arabinitol/L-arabinitol (DA/LA) ratio in diagnosing invasive candidiasis. Ten evaluable febrile neutropenic patients had proven invasive candidiasis and elevated DA/LA ratios were found in 5. Invasive candidiasis with normal DA/LA ratios was most frequent

Escherichia coli P fimbriae utilize the Toll-like receptor 4 pathway for cell activation

Fimbriae mediate bacterial attachment to host cells and provide a mechanism for tissue attack. They activate a host response by delivery of microbial products such as lipopolysaccharide (LPS) or through direct fimbriae-dependent signalling mechanisms. By coupling to glycosphingolipid (GSL) receptors, P fimbriae trigger cytokine responses in CD14 negative host cells. Here we show that P fimbriae ut

Agostic interaction in the methylidene metal dihydride complexes H2MCH2 (M = Y, Zr, Nb, Mo, Ru, Th, or U)

Multiconfigurational quantum chemical methods (complete active space self-consistent field (CASSCF)/second-order perturbation theory (CASPT2)) have been used to study the agostic interaction between the metal atom and H(C) in the methylidene metal dihydride complexes H2MCH2, where M is a second row transition metal or the actinide atoms Th or U. The geometry of some of these complexes is highly ir

Judges', lay judges', and police officers' beliefs about factors affecting children's testimony about sexual abuse

The authors examined Swedish judges', lay judges', and police officers' beliefs about factors that may complicate or facilitate children's reports of sexual abuse. Participants (N = 562) rated potential complicating and facilitating factors and freely reported which criteria they considered important when assessing the reliability of child witnesses. The groups had similar opinions regarding which

Users on the Agenda - Strategies and Theories

This chapter highlights user perspectives related to the development of ICT and the launching of political strategies to involve users in the IT society. Strategies are discussed in relation to cooperation between technical and social scientists. The author points to the fact that the diffrences between various theoretical understandings of the interaction between technology and social change tend

Two insulin gene single nucleotide polymorphisms associated with type 1 diabetes risk in the Finnish and Swedish populations

We have developed high-throughput tests for the detection of the insulin gene region SNPs -23HphI and -2221MspI. The potential of these markers to enhance the efficiency of type I diabetes risk screening was then evaluated by analyzing them in Finnish and Swedish populations. Blood spots on filter paper were analyzed using PCR followed by sequence- specific hybridization and time-resolved fluorome

Immigrants from the Middle-East have a different form of Type 2 diabetes compared with Swedish patients.

AIMS: To compare the clinical characteristics of Type 2 diabetes (T2DM) between immigrants from the Middle-East and Swedish patients. METHODS: The study group included 450 consecutive patients with T2DM, 379 Swedish-born aged 61 +/- 12 years and 71 patients originally from the Middle-East aged 50 +/- 11 years from the diabetes clinic of Malmo University Hospital. RESULTS: Onset of diabetes had occ

Hepatocyte growth factor in cerebrospinal fluid differentiates community-acquired or nosocomial septic meningitis from other causes of pleocytosis.

Due to anatomical restrictions, the inflammatory response to intracerebral bacterial infections exposes swollen brain tissues to pressure and ischemia, resulting in life-threatening damage. Rapid diagnosis and immediate empirical antibiotic therapy is highly important. However, diagnosing meningitis in patients after neurosurgery is complicated, due to brain tissue damage and changes in cerebrospi

Light-induced immobilization of biomolecules as an attractive alternative to micro-droplet dispensing-based arraying technologies

The present work shows how UV light-induced molecular immobilisation (LIMI) of biomolecules onto thiol reactive surfaces can be used to make biosensors, without the need for traditional microdispensing technologies. Using LIMI, arrays of biomolecules can be created with a high degree of reproducibility. This technology can be used to circumvent the need for often expensive nano/microdispensing tec

mzML - a community standard for mass Spectrometry data

Mass spectrometry is a fundamental tool for discovery and analysis in the life sciences. With the rapid advances in mass spectrometry technology and methods, it has become imperative to provide a standard output format for mass spectrometry data that will facilitate data sharing and analysis. Initially, the efforts to develop a standard format for mass spectrometry data resulted in multiple format

On Feminist Activist Aesthetics

This paper discusses some trends in critical theory and activist aesthetics in contemporary feminist performing arts in Sweden. The 2000s have witnessed at least two “turns” in feminist theory, namely the affective turn and the social, or as it is called here, the solidarity turn. The status of poststructuralist theory has been widely debated and Marxist, class-based analyses have returned to the

Cognitive-behavioral therapy for PTSD in children and adolescents: A preliminary randomized controlled trial.

Objective: To evaluate the efficacy of individual trauma-focused cognitive-behavioral therapy (CBT) for treating posttraumatic stress disorder (PTSD) in children and young people. Method: Following a 4-week symptom-monitoring baseline period, 24 children and young people (8Y18 years old) who met full DSM-IV PTSD diagnostic criteria after experiencing single-incident traumatic events (motor vehicle

Ozone depletion and effects of ultraviolet radiation

Because of the stratospheric ozone depletion that took place during the last decades of the past century, the effects of ultraviolet radiation on plants, animals, humans and micro-organisms became intensively studied. In this chapter we describe the role of the ozone layer as a radiation shield and various molecular and organismal effects of ultraviolet radiation, with an emphasis on the UV-B band

Unshrouded rotor tip clearance effects in expander cycle turbines

Steady flow in axial one-stage turbines is assessed numerically and experimentally. The simulations are performed on coarse meshes using a standard numerical approach (3D, steady state, kε-turbulence model, wall function at solid boundaries). In order to allow for conclusions drawn from these rapid numerical studies, the approach was compared with an explicit LDA (Laser Doppler anemometry) mapping