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Purification and characterization of radiolabelled biosynthetic human insulin from Escherichia coli kinetics of processing by antigen presenting cells

An Escherichia coli strain transfected with a plasmid containing four linked human proinsulin genes was grown in the presence of 35S and 3H labelled amino acids to gain access to human insulin that was radiolabelled at 19 evenly distributed sites throughout the amino acid sequence. The multi-proinsulin precursor was cleaved at methionine residues with cyanogen bromide, then the individual proinsul

Organized Self-help Housing: lessons from practice with an international perspective

There is an urgent need of improving the lives of 100 million slum dwellers until year 2020. The Habitat Agenda has identified self-help housing among other enabling housing strategies. This paper focuses on mapping organizations and different types of organized self-help housing (OSHH) projects since year 2000 with the aim of analyzing important lessons from practice. An international survey was

The International Active Learning Space

For me, internationalisation is a learning outcome, not just about international mobility. It is about ensuring that students actively participate in a learning experience that prepares them for a world that is more and more internationally and interculturally connected - that both Danish and non-Danish students receive the basic international and intercultural skills and knowledge they need in cu

Nordics in Motion : Transimperial Mobilities and Global Experiences of Nordic Colonialism

This special issue investigates Nordic individuals in transimperial spaces. It tracks Nordics on the move from the early modern period to the twentieth century, as they crossed imperial boundaries, and connected, networked, and operated in different spaces around the world within the framework of European global expansion. Though coming from countries with few or no formal colonies of their own, N

Introduction : Digital Methods and Experiential Approaches to the Past

In this chapter, the authors introduce the theme of computational/digital methods in the realm of sensory archaeologies. To this purpose, the concept of sensoriality and its relevance for the archaeological discourse is rediscussed. Key concepts such as perception of material culture help us in better defining possible modalities through which archaeologists can benefit from the use of formal meth

Does fatigue in Parkinson’s disease impact one’s physical function in daily live?

Objective: To investigate fatigue at baseline and 3.5 years later and its association with balance and gait over time.Background: In Parkinson’s disease (PD), fatigue is considered one of the most common and disabling nonmotor symptoms that may persist or even worsen over time. Fatigue in generally has been described as a significantly diminished energy level or an increased perception of effort d

Affibody molecules for PET imaging

Optimization of Affibody molecules would allow for high contrast imaging of cancer associated surface receptors using molecular imaging. The primary aim of the thesis was to develop Affibody-based PET imaging agents to provide the highest possible sensitivity of RTK detection in vivo. The thesis evaluates the effect of radiolabelling chemistry on biodistribution and targeting properties of Affibod

Long-term outcomes following adolescent metabolic and bariatric surgery

Severe obesity in adolescence negatively impacts upon health and wellbeing. Lifestyle modifications do not usually achieve a sufficient degree or durability of weight loss to mitigate the risk of medical complications. In recent years, metabolic and bariatric surgery (MBS), already a well-established treatment for adults with severe obesity, has emerged as an option in adolescents. Controlled stud

Car free urban districts – Interactions between measures, implementation and experience

Cities and car traffic does not always fit well together. Apart from the well-knownenvironmental problems associated with traffic congestion (e.g. air pollution and noise,climate change and global warming), there are also negative local effects in the form ofincreased risk of traffic accidents, abuse of key resources (energy and time), gender issues interms of accessibility, security and power ove

The Future Is (Ancient) History : Judge Dredd and the Futuristic Legacy of the Classical World

The article explores how the legacy of ancient Rome served the creative vision of the future found in the british comic Judge Dredd, firstpublished in 1977. The article argues that Judge Dredd appropriated motifs from Roman history to help establish the framework of the future and that the comic’s satirical tone was aided by these allusions to the classical world. The article concludes that the in

3 things they don't tell you about star clusters

Dense stellar systems in general and star clusters in particular have recently regained the interest of the extragalactic and even cosmology communities, due to the role they could play as actors and probes of re-ionization, galactic archeology and the dark matter content of galaxies, among many others. In the era of the exploitation and the preparation of large stellar surveys (Gaia, APOGEE, 4MOS