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Inhibition of denitrification and oxygen utilization by Thiosphaera pantotropha

Mass spectrometry confirmed that the entire denitrification system of Thiosphaera pantotropha is present under aerobic conditions. Acetate-dependent oxygen uptake is totally inhibited by 0.3mM cyanide. Nitrate reduction by cells grown with and without nitrate was inhibited by 10 and 15mM azide, respectively. The nitrous oxide reductase was totally inhibited by 1% acetylene or 0.125mM cyanide. This

Molecular origin of time-dependent fluorescence shifts in proteins

Time-resolved fluorescence spectroscopy is used increasingly to probe molecular motions at the aqueous interfaces of biological macromolecules and membranes. By recording the time variation of the fluorescence frequency, thermal atomic fluctuations in the vicinity of the chromophore can be probed. From such fluorescence Stokes shift (FSS) experiments, it has been inferred that water motions in the

Antibody microarrays: Current status and key technological advances

Antibody-based microarrays are among the novel classes of rapidly evolving proteomic technologies that holds great promise in biomedicine. Miniaturized microarrays (< 1 cm(2)) can be printed with thousands of individual antibodies carrying the desired specificities, and with biological sample (e.g., an entire proteome) added, virtually any specifically bound analytes can be detected. While consumi

The kinetics of leucocyte migration into rabbit knee joints elicited by preformed immune complexes with different in vitro characteristics

Immune complexes were formed in vitro with antibodies obtained from rabbits immunized with bovine serum albumin in Freund's complete (FCA) or incomplete (FIA) adjuvant. The antibodies were mixed with different amounts of antigen. Immune complexes formed at maximum precipitation proportions were efficient in complement activation, whereas immune complexes formed at antigen excess had weak complemen

Metastable entangled states of atomic systems in macroscale: radiation dynamics and spectrum

Dynamics of spontaneous decay and corresponding emission spectrum areexactly treated for a system of two identical two-level atoms in 1D.Just a one-dimensional case is considered in order to maximally extractan information on mutual influence of spatially remote atoms through theradiation field. As a result, an effect is revealed and considered indetail that there exist stable (metastable) entangl

Dendritic Spine Instability Leads to Progressive Neocortical Spine Loss in a Mouse Model of Huntington's Disease.

In Huntington's disease (HD), cognitive symptoms and cellular dysfunction precede the onset of classical motor symptoms and neuronal death in the striatum and cortex by almost a decade. This suggests that the early cognitive deficits may be due to a cellular dysfunction rather than being a consequence of neuronal loss. Abnormalities in dendritic spines are described in HD patients and in HD animal