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Detection of Pseudomonas aeruginosa infection using a sustainable and selective polydopamine-based molecularly imprinted electrochemical sensor

Pyocyanin, a redox-active secondary metabolite produced by Pseudomonas aeruginosa, serves as a crucial virulence factor. Detection and quantification of pyocyanin can aid early diagnosis of infection. A selective and sensitive molecularly imprinted electrochemical sensor was constructed by using a green polymerization technique to deposit an ultrathin polydopamine film on an electrode modified wit

The ubiquity and type diversity of papillomaviruses in normal skin

Popular Abstract in Swedish Papillomvirus infekterar ett brett spektrum av ryggradsdjur, såsom människa, olika ap-arter, ko, älg, elefant, ren och bofink. Dessa virus kan ge så vitt skilda symptom som cancer och vårtor. De indelas efter vilken art de infekterar, och inom arterna efter skillnad i DNA-sekvens mellan olika isolat (typer). Hittills har 92 humana papillomvirus (HPV) typer beskrivits, oPapillomaviruses are a diverse, epitheliotropic group of viruses. Some genotypes are the main cause of anogenital cancer in humans; others can induce skin lesions. In order to investigate the occurrence of subclinical skin infections with human papillomavirus (HPV), a pair of degenerate primers (FAP59/64) was designed. Moist cotton-tipped swabs were used as a gentle method of sample collection. Di

Post-translational events in proteoglycan synthesis : Kinetics of synthesis of chondroitin sulfate and oligosaccharides on the core protein

Chondrocytes isolated from the Swarm rat chondrosarcoma were incubated in culture with [1-3H]glucose for 30 min to 8 h. Labeled proteoglycans were isolated, treated with borohydride under alkaline conditions, and the three complex sugar structures purified: N- and O-linked oligosaccharides and chondroitin sulfate chains. The amount of incorporated radioactivity into each component sugar was analyz

Workload-study of ISA-drivers - A method description.

On the decision of the Swedish government, the Swedish National Road Administration (SNRA) has started a large-scale trial with Intelligent Speed Adaptation (ISA) in urban areas. One of the four test sites is the city of Lund. At the Department of Technology and Society, Lund University, research about ISA has been carried out since 1986 and the first field study was carried out in 1993. The ongoi

Spatial and temporal variability of land CO2 fluxes estimated with remote sensing and analysis data over western Eurasia

The Eurosiberian Carbonflux project was designed to address the feasibility of inferring the regional carbon balance over Europe and Siberia from a hierarchy of models and atmospheric CO2 measurements over the continent. Such atmospheric CO2 concentrations results from the combination of connective boundary layer dynamics, synoptic events, large-scale transport of CO2, and regional surface fluxes

A population-based study of the outcome for patients with first relapse of Hodgkin's lymphoma

Background : Our aims were to evaluate the response to salvage treatment in relation to initial treatment and to evaluate prognostic factors at the time of relapse in an unselected population of relapsing patients with Hodgkin's lymphoma (HL). Patients and methods: In total, 124 patients younger than 60 yr of age with initial diagnosis of HL in Sweden relapsed between 1985 and 1995. Results : Fift

N-glycans and the N terminus of protein C inhibitor affect the cofactor-enhanced rates of thrombin inhibition

Protein C inhibitor (PCI) is a serine protease inhibitor, displaying broad protease specificity, found in blood and other tissues. In blood, it is capable of inhibiting both procoagulant and anticoagulant proteases. Mechanisms that provide specificity to PCI remain largely unrevealed. In this study we have for the first time provided a full explanation for the marked size heterogeneity of blood-de

Chemo-enzymatic epoxidation of linoleic acid: Parameters influencing the reaction

The effect of reaction parameters on lipase-mediated chemo-enzymatic epoxidation of linoleic acid was investigated. Hydrogen peroxide was found to have the most significant effect on the reaction rate and degree of epoxidation. Excess of hydrogen peroxide with respect to the amount of double bonds was necessary in order to yield total conversion within a short time period, as well as at temperatur

Reducing costs, keeping high quality Passive Houses by use of BIM and prefabrication

The construction of Passive Houses is challenging. On one hand, there is a strong pressure to reduce the investment cost and on the other hand optimal energy efficiency should be reached. Hence that it is indispensable for architects, engineers, and constructors to avoid any causes throughout the whole project, that would lead to performance gaps and costs overruns. The purpose of this degree proj

Analyzing V2V signal models in simulated 3D geometry-based stochastic channels

Vehicle to everything (V2x) communication is one of the most researched topics today. The main goal behind this new emerging technology is road safety along with traffic and energy efficiency. Vehicular communications can further be sub-divided into certain types. One such type is vehicle to vehicle (V2V) communication, which is the focus of this thesis. V2V is either Wireless Local Area Network (

Soil, Sand and City

Israel utropades formellt som en oberoende stat 1948, en tid när den modernistiska ideologin nådde sin kulmen globalt även fast det haft ett stadigt grepp om stadsplaneringens normer sedan tidigt 1920-tal. Det stora antalet migranter som under kort tid bosatte sig i den nybildade nationen resulterade i ett behov av snabb stadsutveckling. Den stora tillströmningen av människor attraherade dessutom Israel was officially declared as an independent state in 1948. A time when the modernistic ideology peaked around the globe, although it had been the dominant urban planning paradigm since the early 1920s. The large number of migrants moving to the newly formed nation stressed the need of rapid urban development. The large influx of people also attracted many planners and architects from the west

Effektiva kvadratmeter - program och koncept för studentlägenheter

Student housing is an ongoing and ever debated issue. Students often have to pay for inefficient square meters which are both difficult to furnish and lack function. In Helsingborg, the supply of student housing is wide but with varying quality and efficiency. Therefore, future student accommodations in Helsingborg should be carefully planned and adapted to their purpose - with more effective squa

Mitigating Urban Heat Island effects in Beirut

Urbanisation in Beirut has led to a situation where the city suffers from a severe lack of green public space—Lebanon’s capital have ended up with some of the least amount of public green space per capita in the world. The lack of green surfaces and vegetation in Beirut contributes to the city’s Urban Heat Island (UHI)—a phenomenon where an urban or metropolitan area traps waste heat and end up si

Spatio -Temporal Modelling of Air Pollution in Malta

Air pollution has become a major worldwide concern due to the high levels of air pollutants emitted from industrial and traffic related activities. Exposure to air pollution has been linked to various negative health effects, ranging from asthma to chronic illnesses. Consequently, analyzing air pollution data has become an essential tool for giving insight about the potential health effects. Spati

Civilprocessuella säkerhetsåtgärder i entreprenadtvister - En analys av civilprocessuella säkerhetsåtgärder som ett medel för att säkerställa entreprenadens framdrift

I entreprenadprojekt finns det en risk för att entreprenören av någon anledning avbryter eller befaras avbryta arbetet. En eventuell domstolsprocess avseende fullgörelse av entreprenadavtalet riskerar dock att bli tidsödande och hindra projektets framdrift. Det ligger därför i beställarens intresse att entreprenören, genom förordnande om civilprocessuell säkerhetsåtgärd enligt 15 kap. 3 § RB, kan There is a risk that a contractor discontinues or plans to discontinue the work during construction. Potential court proceedings regarding the performance of the construction contract could be time-consuming and delay the whole project. Therefore, it is in the customer’s interest that the contractor, by a security measure (preliminary injunction), can be required to continue the contract work or p

The natural course of spontaneous osteonecrosis of the knee (SPONK) A 1-to 27-year follow-up of 40 patients

Background and purpose Spontaneous osteonecrosis of the knee (SPONK) is a painful lesion in the elderly, frequently leading to osteoarthritis and subsequent knee surgery. We evaluated the natural course and long-term consequences of SPONK in terms of need for major knee surgery. Methods Between 1982 and 1988, 40 consecutive patients were diagnosed with SPONK. The short-term outcome has been report

The role of higher-order protein structure in supporting binding by heteroclitic monoclonal antibodies: The monoclonal antibody KIM185 to CD18 also binds C4-binding protein

Heteroclitic monoclonal antibodies are characterized by the ability to bind multiple epitopes with little or no similarity. Such antibodies have been reported earlier, but insight into to the molecular basis of this propensity is limited. Here we report that the KIM185 antibody to human CD18 reacts with the plasma protein C4b-binding protein (C4BP). This was revealed during affinity purification p

Specific (CD2CD2SCHD2)-C-12-D-beta-C-12-D-gamma-C-13-H-epsilon Isotopomer Labeling of Methionine To Characterize Protein Dynamics by H-1 and C-13 NMR Relaxation Dispersion

Protein dynamics on the micro- to millisecond time scale is increasingly found to be critical for biological function, as demonstrated by numerous NMR relaxation dispersion studies. Methyl groups are excellent probes of protein interactions and dynamics because of their favorable NMR relaxation properties, which lead to sharp signals in the H-1 and C-13 NMR spectra. Out of the six different methyl