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The Design and Structure Prediction of Protein Oligomers

The minimum free energy state of a protein (the native state) is encoded by its amino-acid sequence. Due to the many torsional degrees of freedom (DOF) available to a polypeptide chain, a vast number of conformations is possible. Therefore, to predict the native state of a protein directly from sequence, a computer algorithm must evaluate a large number of possible conformations using accurate sco

Perdeuteration of Biological Macromolecules: A Case Study of Human Carbonic Anhydrases

Deuterated biomolecules - where hydrogen atoms (H) are exchanged to its isotope deuterium (D) - are essential for biological experiments in neutron scattering, but they are not readily available. In neutron scattering, D has a strong positive coherent scattering length (6.67 fm) in comparison with the negative scattering length of H (-3.74 fm). These different characteristics of neutrons to D and

Molecular development of a thermostable β-glucosidase for modification of natural products

Popular Abstract in English The gene encoding a β-glucosidase originating from the extreme thermophile Thermotoga neopolitana has been cloned and expressed in Escherichia coli. The aim was to produce a thermostable enzyme that could be used to remove sugar residues from glucosylated natural products classified as flavonoids by applying a method combining extraction in hot pressurized water with enThe gene encoding a β-glucosidase originating from the extreme thermophile Thermotoga neopolitana has been cloned and expressed in Escherichia coli. The aim was to produce a thermostable enzyme that could be used to remove sugar residues from glucosylated natural products classified as flavonoids by applying a method combining extraction in hot pressurized water with enzymatic hydrolysis. The β-g

CSR Communication: An Explorative Study on the Message Content and the Meanings they Convey

We explored how a company tries to overcome the problems of awareness, attribution and the definitional misunderstandings of the CSR concept by investigating the message content of communication of a company that positions themselves on their CSR values and activities. This study has an exploratory approach with a strong qualitative focus. The empirical data for this study was collected from Baris

Baeyer-Villiger Monooxygenases from a Dietzia sp. - Enzyme Discovery, Characterization and Engineering

With the emergence of Green Chemistry, biocatalysis is becoming an important approach in many laboratory and industrial processes. Enzymes catalyse chemical reactions with high regio- and stereoselectivity at mild conditions and, most importantly, are able to form products that are not possible to obtain by conventional synthetic chemistry. Monooxygenases are a fascinating group of enzymes which o