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Sarcomeric mitochondrial creatine kinase (Mi(b)-CK) of chicken was expressed in Escherichia coli as a soluble enzyme by using an inducible phage-T7 promoter. Up to one third of the protein in E. coli extracts consisted of soluble recombinant Mi(b)-CK in an enzymically active form. Approx. 20 mg of nearly-homogenous Mi(b)-CK was isolated in a two-step isolation procedure starting with 1 litre of is
Aim: The aim of this pilot study was to explore the Fear of Birth Scale in a mixed sample of women of childbearing age, by investigating the levels of childbirth fear and the content of women's thoughts when completing the scale. Methods: A cross-sectional mixed method study of 179 women who completed a short questionnaire and a think aloud interview. Results: The mean score of the Fear of Birth S
Arctic homeotherms counter challenges at high latitudes using a combination of seasonal adjustments in pelage/plumage, fat deposition and intricate thermoregulatory adaptations. However, there are still gaps in our understanding of their thermal responses to cold, particularly in Arctic birds. Here, we have studied the potential use of local heterothermy (i.e. tissue cooling that can contribute to
Public procurement represents a very large share of most economies worldwide. Besides its direct purchasing power, public procurement has an enormous potential to become one of the most important mission-oriented policy instruments in the context of the Sustainable Development Goals. The paper argues that the key to achieve more innovations when pursuing public procurement is to describe problems
This doctoral thesis uses a meta-critical method with an epistemological approach. The corpus consists of the doctoral theses presented in the United States about the novels of the Peruvian-Spanish writer and Nobel Prize winner in 2010 Mario Vargas Llosa, one of the most outstanding Spanish-speaking authors, with an extensive literary production. The study covers the 56 doctoral theses presented b
Background:: Strategies for the involvement of primary care in the management of patients with presumed or diagnosed dementia are heterogeneous across Europe. We wanted to explore attitudes of primary care physicians (PCPs) when managing dementia: (i) the most popular cognitive tests, (ii) who had the right to initiate or continue cholinesterase inhibitor or memantine treatment, and (iii) the rela
Background and purpose — The number of patients where shoulder hemiarthroplasty (SHA) is an option is still substantial. Descriptive analyses performed by the Swedish Shoulder Arthroplasty Registry (SSAR) showed that while patients receiving SHA designs, i.e. resurfacing hemi (RH) and stemmed hemi (SH), reported similar shoulder functionality and quality of life, the revision rate for RH (12%) was
Background: This paper describes the background, aim and study design for the Swedish SELMA study that aimed to investigate the importance of early life exposure during pregnancy and infancy to environmental factors with a major focus on endocrine disrupting chemicals for multiple chronic diseases/disorders in offspring. Methods: The cohort was established by recruiting women in the 10th week
The objective of this study was to estimate the tensile strength of surgical knots made using the da Vinci robot. Four different types of flat square knots (strand-to-strand 4 throw, strand-to-strand 6 throw, loop-to-strand 4 throw, and loop-to-strand 6 throw) were made using the da Vinci-S system by 4 different surgeons, all experienced with the system. For the knots, we used braided polyglactin
We and others have previously shown that inhibitor containing plasma from patients with congenital haemophilia A sometimes reacts less with von Willebrand factor (VWF) containing concentrates compared with highly purified plasma-derived or recombinant factor VIII (FVIII) concentrates. To further substantiate the haemostatic role of a variation in inhibitor reactivity with different FVIII concentra
BACKGROUND AND AIM: Persistent organochlorine pollutants (POPs) have in epidemiological studies been associated with health hazards. The exposure window might be crucial and it is, accordingly, important to analyze exposure concentrations in samples collected at the window of interest. This makes the use of stored blood samples extremely interesting. However, small blood volumes from stored sampl
PURPOSE: The aim of this study was to explore healthcare consumption in relation to more versus less knowledge concerning prescribed drugs among older people with functional dependency and repeated healthcare contacts, and to explore the determinants of more versus less knowledge METHODS: The sample comprised 63 persons (mean age 82.8 years). Data concerning use and knowledge about drugs, demograp
Standing on a foam surface is believed to exaggerate balance deficits by decreasing the reliability of somatosensory information from cutaneous mechanoreceptors on the plantar soles (i.e. base of feet) and by altering the effectiveness of ankle torque. The aim was to further document the nature of foam posturography testing by comparing between standing on foam and standing with decreased Rapidly
The magnitude of variation in the gaze position signals recorded by an eye tracker, also known as its precision, is an important aspect of an eye tracker’s data quality. However, data quality of eye-tracking signals is still poorly understood. In this paper, we therefore investigate the following: (1) How do the various available measures characterizing eye-tracking data during fixation relate to
Sex pheromone biosynthesis in the codling moth Cydia pomonella (Lepidoptera; Tortricidae) was studied by topical application of deuterated fatty acids in DMSO to pheromone glands. The incorporation of deuterium label into fatty acids and alcohols in the pheromone gland was monitored by gas chromatography with flame ionization detection and mass spectrometry in the selected ion monitoring mode. Dod
On June 13, 2013, the U.S. Supreme Court decided the Myriad gene patent case. In a unanimous judgment the Court held that patent claims directed to isolated genomic DNA are identical to the naturally occurring sequence and thus unpatentable “products of nature”. This decision affects all isolated"products of nature", including genes, gene fragments, and other naturally occurring nucleotide sequenc
The present global environmental condition is a consequence of the increasing consumption of natural resources whose depletion exceeds what is physically possible to sustain in the long term. The construction sector is a considerable contributor to this resource depletion and sustainability is adopted in the form of the theory of ecological modernization. The implementation depends to varying exte
Abstract Recent years have seen a number of competing theories of concepts within philosophy of mind, supplanting the classical definitionist and imagist accounts: among them, Jerry Fodor's informational atomism, Jesse Prinz's proxytypes theory, and -- of course -- Peter Gärdenfors' (2004) conceptual spaces theory (CST). On the whole there has been little empirical investigation into the competing
Before the minimum tax directive (2003/96/EC) took effect in 2004, Swedish industries had enjoyed untaxed electricity for over a decade. While the introduction of the tax increased costs for many companies, energy intensive industries were eligible for exemption if they entered an agreement on energy efficiency. Sweden quickly implemented the directive and simultaneously launched the Programme for
The fight against antibiotic resistance is one of the most significant challenges to public health of our time. The inevitable development of resistance following the introduction of novel antibiotics has led to an urgent need for the development of new antibacterial drugs with new mechanisms of action that are not susceptible to existing resistance mechanisms. One such compound is HAMLET, a natur
