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Overproduction of membrane proteins can be a cumbersome task, particularly if high yields are desirable. NADH:quinone oxidoreductase (complex I) contains several very large membrane-spanning protein subunits that hitherto have been impossible to express individually in any appreciable amounts in Escherichia coli. The polypeptides contain no prosthetic groups and are poorly antigenic, making optimi

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Protein kinase B (PKB)/Akt has been strongly implicated in the insulin-dependent stimulation of GLUT4 translocation and glucose transport in skeletal muscle and fat cells. Recently an allosteric inhibitor of PKB (Akti) that selectively targets PKB alpha and -beta was reported, but as yet its precise mechanism of action or ability to suppress key insulin-regulated events such as glucose and amino a

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Syftet med uppsatsen är att reda ut förekomsten av intertextuella och intratextuella referenser i Twin Peaks. Med detta menas de filmlanskaps som kommenteras i serien Jag försöker i texten kartlägga deras härstamning, beskriva hur de dyker upp i serien och förklara deras förekomst. Till sist avser jag att ställa referenserna i relation till Lynchs bakgrund. Även denna utgör en fond mot vilken seri

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Large-scale restoration projects are being increasingly used to address land degradation and global climate change. While these initiatives are promising, an overemphasis on tree planting may contribute to ecological degradation and carbon loss. This thesis critically examines the AFR100 initiative in southern Africa through literature analysis and expert interviews. By integrating perspectives fr

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In the offshore industry, reliability of installed equipment is of paramount concern due to safety, environmental and economic consequences. The costs of unplanned shutdowns and maintenance have high economic implications and are of increasing importance with tighter profit margins. Reliability is therefore of significance when selecting equipment model and brand. This report presents a survey of

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It is frustrating for every reasonable human being to observe scenes of exploitation around the world in respect of children, because of their either naivety or vulnerability. It is unacceptable that still in the 21st century, children do not benefit from the protection, attention and love they deserve. An estimated 300 million children worldwide are subject to violence, exploitation and abuse inc

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Sammanfattning Titel: Dags fo¨r ett smartare retail – En studie i hur den smartphoneanva¨ndande kunden bemo¨ts av butiken. Universitet: Lunds Universitet, Campus Helsingborg, Institutionen fo¨r Service Management. Kurs: SMKK01 Service Management: Examensarbete kandidatexamen, 15 Hp, VT2012. Seminariedatum: 29 maj 2012. Fo¨rfattare: Yichu Chung, Andreas Nilsson och Johanna Westerdahl. Handledare: B

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This report covers a survey of the natural occurence of the heavy metal cadmium (Cd) in soil and bedrock in Skåne, Sweden and Europe. The main focus is on the cadmium concentrations in samples of sphalerite (ZnS) collected in quarries in Skåne during the study, and in samples of sphalerite from the rest of Sweden and locations in Spain and USA depostited by the Department of Geology, Lund Universi

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A growing world population confronts humanity and the planet with several challenges due to its increased demand for food, space and energy, implying changes in land use. While agriculture currently provides enough food, it is destroying biodiversity and hence aggravates the planet ́s vulnerability to climate change. A paradigm shift might therefore be needed. Applying a political ecology lens, co

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The present contribution focuses on the mathematical techniques used to solve steady state metabolic models for the case of an overdetermined system. Even when parts of the system are underdetermined it is possible to solve the model partially and obtain statistically meaningful results. This is illustrated with data gathered from a set of E. coli W3110.shik1 phosphate- or carbon-limited continuou

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BACKGROUND: The dual-mobility acetabular cup (DMC) has an additional bearing consisting of a mobile polyethylene component between the prosthetic head and the outer metal shell. This design has gained popularity in revision total hip arthroplasty (THA) and in primary treatment of femoral neck fractures with the anticipation of a reduced risk of THA instability. Our primary aim was to evaluate the

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Urethral obstruction in cats is an emergency that without adequate intervention may progress to uremia and death of the patient. Prolonged distension of the bladder will result in loss of contractile function and consequently, bladderatonia and this can become permanent. It is concluded that the pre-pubic urethrostomy in feline is effective for correction of urethral stenosis even afte previous pe

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BACKGROUND: Given today's high prevalence of common mental disorders and related sick leave among teachers, an urgent need exists for a more systematic approach to the management of social and organizational risk factors within schools. In 2015, we launched the first Swedish occupational health guideline to support a structured prevention of these risks at the workplace. The existence of guideline

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Background When the lectinlike oxidized low-density lipoprotein (ox LDL) receptor-1 ( LOX -1), a scavenger receptor for ox LDL , binds ox LDL , processes leading to endothelial dysfunction and inflammation are promoted. We aimed to study release mechanisms of LOX -1 and how circulating levels of soluble LOX -1 ( sLOX -1) relate to plaque inflammation and future risk for ischemic stroke. Methods an

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This study focuses on drivers for changing urban flood risk. We suggest a framework for guiding climate change adaptation action concerning flood risk and manageability in cities. The identified key drivers of changing flood hazard and vulnerability are used to provide an overview of each driver's impact on flood risk and manageability at the city level. We find that identified drivers for urban f

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Searches for dijet resonances with sub-TeV masses using the ATLAS detector at the Large Hadron Collider can be statistically limited by the bandwidth available to inclusive single-jet triggers, whose data-collection rates at low transverse momentum are much lower than the rate from standard model multijet production. This Letter describes a new search for dijet resonances where this limitation is

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A search for dark matter produced in association with a Higgs boson decaying to b-quarks is performed using pp collisions at a centre-of-mass energy of √s=13 TeV. The dataset has an integrated luminosity of 79.8 fb−1 and was recorded with the ATLAS detector at the Large Hadron Collider. Selected collision events comprise large missing transverse momentum and either two b-tagged small radius jets o