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Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however
The β-cell function, total islet volume, and number were studied in 1- to 18-month-old mice, together with the extractable pancreatic insulin and glucagon. The β-cell function, determined as the total amount of insulin released in response to glucose from the in vitro perfused pancreas showed an agerelated increase, without any differences in the kinetics of insulin secretion between young and old
ABSTRACT The pancreatic B‐cell function (glucose tolerance, C‐peptide release) and organ‐specific autoantibodies, including islet cell cytoplasmic and cell surface (mouse), were studied in 45 first‐degree relatives of patients with insulin‐dependent diabetes mellitus diagnosed before the age of 30 years. Compared to 107 healthy persons without any family history of either insulin‐dependent or non‐
The probable role of cell surface antigens in the development of juvenile diabetes necessitates a detailed study of the proteins expressed on the surface of pancreatic β-cells. A gene library composed of genes expressed in β-cells was constructed to provide a source of low abundance genes from those cells. Initial studies with this library have led to the isolation of three genes which share nucle
Litters of BB rats with an expected high and low incidence of insulin-dependent diabetes were followed from weaning until the age of about 140 days. Islet cell surface antibodies (ICSA) and lymphocyte antibodies (LA) were determined in a radioligand assay with fixed rat insulinoma (RIN 5F) cells, an insulin-secreting cell line, or spleen lymphocytes. In the low-incidence litter, 2 out of 14 rats h
An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of Mr36 000 and a minor protein of Mr 58 000 from human pancreatic islets. The biosynthetic incorporation of [35S]methionine into the 36-kDa protein increased 2-3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or with
The sensitivity and specificity of the assay for islet cell cytoplasmic antibodies in human serum were examined using cryostat sections from fresh frozen pancreas. The specificity of the assay was close to 100% while the sensitivity was 40%-98% depending on the pancreas used. Inter-observer variation was 12-27%. End-point titres of islet cell antibodies varied with the sensitivity of each pancreas
Non-competitive N-methyl-D-aspartate receptor antagonists mimic schizophrenia symptoms and produce immediate and persistent antidepressant effects. We investigated the effects of ketamine and phencyclidine (PCP) on thalamo-cortical network activity in awake, freely-moving male Wistar rats to gain new insight into the neuronal populations and brain circuits involved in the effects of NMDA-R antagon
The human HLA-D histocompatibility region encodes class II antigens each of which consists of two polypeptide chains (α and β) inserted in the plasma membrane. These molecules are implicated in the regulation of the immune response1-3 but several human diseases are also found to be associated with certain HLA-DR antigens4. The occurrence of insulin-dependent (type I) diabetes (IDDM) is strongly as
A quantitative method to measure islet cell surface antibodies in human patients has been developed using 125I-protein A. Isolated, dispersed, viable rat islet cells prepared by collagenase digestion were fixed in 4% paraformaldehyde to allow storage for up to 7 wk at 4°C. Human sera, heat inactivated and adsorbed with rat liver and kidney powder (100 mg/ml), were incubated with the fixed cells (5
Glaciärer är väldigt effektiva på att forma landskapet och skapa nya sediment och landformer genom erosion, deposition och deformation. Genom att studera dessa landformer och sediment kan värdefull information erhållas om de processer som formade dem, glaciärers historia samt klimat- och miljöförändringar.Detta projekt fokuserar på glaciala landformer och sediment i den lägre-Borgarfjörður regioneLarge and complete sections through glacial landforms formed in subaqueous environments are rare, impeding our knowledge of their internal composition and the processes responsible for their formation. Following the last deglaciation of the Icelandic Ice Sheet (IIS), a marine-terminating outlet glacier advanced out of the fjord of Borgarfjörður resulting in large-scale glaciotectonic deformation o
An improved value for the lifetime of the (anti-)hypertriton has been obtained using the data sample of Pb–Pb collisions at sNN=5.02 TeV collected by the ALICE experiment at the LHC. The (anti-)hypertriton has been reconstructed via its charged two-body mesonic decay channel and the lifetime has been determined from an exponential fit to the dN/d(ct) spectrum. The measured value, τ=242−38 +34 (sta
The jet radial structure and particle transverse momentum (pT) composition within jets are presented in centrality-selected Pb–Pb collisions at sNN=2.76 TeV. Track-based jets, which are also called charged jets, were reconstructed with a resolution parameter of R=0.3 at midrapidity |ηch jet|<0.6 for transverse momenta pT,ch jet=30–120 GeV/c. Jet–hadron correlations in relative azimuth and pseudora
BACKGROUND: Type 2 diabetes (T2D) is a complex polygenic disease with unclear mechanism. In an attempt to identify novel genes involved in β-cell function, we harness a bioinformatics method called Loss-of-function tool (LoFtool) gene score. METHODS: RNA-sequencing data from human islets were used to cross-reference genes within the 1st quartile of most intolerant LoFtool score with the 100th most
CONTEXT: Metabolomics has the potential to generate biomarkers that can facilitate understanding relevant pathways in the pathophysiology of type 2 diabetes (T2DM). METHODS: Nontargeted metabolomics was performed, via liquid chromatography-mass spectrometry, in a discovery case-cohort study from the Malmö Preventive Project (MPP), which consisted of 698 metabolically healthy participants, of whom
OBJECTIVE: We investigated potential interactions between body mass index (BMI) and genotypes of human leukocyte antigen (HLA), TCF7L2-rs7903146, and FTO-rs9939609 in relation to the risk of latent autoimmune diabetes in adults (LADA) and type 2 diabetes. METHODS: We pooled data from two population-based studies: (i) a Swedish study with incident cases of LADA [positive for glutamic acid decarboxy
Polymer films based on mixtures of ethyl cellulose (EC) and hydroxypropyl cellulose (HPC) have been widely used to coat pellets and tablets to modify the release profile of drugs. For three different EC/HPC films we used 1H and 19F MRI in combination with a designed release cell to monitor the drug, polymer and water in 5 dimensional (5D) datasets; three spatial, one diffusion or relaxation and a
