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In chlorophyll biosynthesis, insertion of Mg2+ into protoporphyrin IX is catalysed in an ATP-dependent reaction by a three-subunit (BchI, BchD and BchH) enzyme magnesium chelatase. In this work we present the three-dimensional structure of the ATP-binding subunit BchI. The structure has been solved by the multiple wavelength anomalous dispersion method and refined at 2.1 A resolution to the crysta

Two monoclonal antibodies (mAb) CB268 and CII-C1 to type 11 collagen (CII) react with precisely the same conformational epitope constituted by the residues ARGLT on the three chains of the CII triple helix. The antibodies share structural similarity, with most differences in the complementarity determining region 3 of the heavy chain (HCDR3). The fine reactivity of these mAbs was investigated by s

Vascular smooth muscle cells (VSMCs) in the intact vascular wall are differentiated for contraction, whereas the response to vascular injury involves transition towards a synthetic phenotype, with increased tendency for proliferation. Platelet-derived growth factor (PDGF) is thought to be important for this process. We investigated expression and functional coupling of PDGF receptors (PDGFRs) alph

The variable region 1 to 3 (V1-V3) of the HIV-1 envelope plays an important role in coreceptor usage. Early in infection HIV- 1 is using CCR5 as coreceptor to enter target cells (R5 viruses) whereas viruses using CXCR4 as coreceptor (X4 viruses) may appear later in infection. This broadening or switch in coreceptor usage is associated with progression to AIDS. In my thesis work, we have compared t

We compared the differences between spring and autumn in migration speed, fuelling rates and fuel loads of migrating Red Knots Calidris canutus. As a basis we used ringing data from Ottenby Bird Observatory, southeastern Sweden, collected 1948–2003, with morphometrical data from 1990–2003. Numbers ringed varied between 0 and 301 per year (average 56). Morphometrics, recoveries and recaptures of ri

Background/Aim: Viral respiratory infections are increasingly implicated in allergic exacerbations. Virus-induced activation of eosinophils through Toll-like receptors (TLRs) could be involved. The present study was designed to examine TLR3 expression in eosinophils from bone marrow (BM) and peripheral blood (PB) during symptomatic allergic rhinitis, and to evaluate the functional responsiveness o

Popular Abstract in Swedish Urininkontinens utgör ett stort socialt problem, framför allt hos den äldre befolkningen. Kliniskt är urininkontinens iakttaget i större omfattning hos kvinnor än hos män, men studier antyder männen är mer drabbade än vad nuvarande statistik gör gällande. Grundat på symptombild kan urininkontinens indelas i trängningsinkontinens, ansträngningsinkontinens samt överrinninNormal urine storage and bladder emptying are controlled by the central (CNS) as well as the peripheral nervous system. Dysfunction of these mechanisms can lead to urinary incontinence. Urinary tract dysfunctions have symptomatically been treated mainly with drugs acting peripherally. However, in the CNS, drug targets for pharmacological intervention may be found. The present study deals with effe

Popular Abstract in Swedish Olika sätt att mäta substrat (monosackarider) och produkter (främst etanol) vid tillverkning av biobränslet etanol har studerats. Etanoltillverkning sker i en fermentor med hjälp av mikroorganismer som kan omvandla socker. Det är av stort intresse att mäta koncentrationen av produkter och substrat under tillverkningsprocessens gång för att veta om den fortlöper som den Two different detection methods for on-line monitoring of substrates and products in ethanol fermentations have been studied. Amperometric biosensors were developed for use as detectors in flow injection or liquid chromatographic (LC) systems. Carbon paste was used as the electrode material and served as the supporting matrix to incorporate the enzymes alcohol oxidase or pyranose oxidase. Hydrogen

Popular Abstract in English Proteins are molecular machines of the human body which regulate all life sustaining processes such as energy production, communication, transport of essential molecules, defense against microbes, etc. They are built by small molecular units called amino acids, bound together like pearls on a necklace. Each amino acid consists of a common part and a unique side chain. TAnisotropic protein interactions have a strong orientation dependence resulting from an uneven distribution of charged and hydrophobic residues on the protein surface. They play an important role in protein behaviors such as protein association, surface adsorption and phase separation. In this thesis, we have studied the effect of anisotropic interactions on the behavior of various proteins mainly

Purpose of this paper The outcome of product development, i.e. the product design, together with the packaging solution, has a great impact on the logistics performance of supply chains. Despite this, packaging has traditionally been given a very modest role in the product development literature as well as in the logistics literature. The aim of this paper is to show how logistics performance an

Two 8-year-old Pinus radiata trees growing in a dry-land plantation forest were subjected to a partial shading treatment for 2 years in order to investigate the responses of photosynthesis and tree growth to a long-term reduction in illuminated leaf area. The lower 60% of the crown of shaded trees was surrounded by 50% neutral density shade cloth. This reduced radiation absorption by 13% for the t

Popular Abstract in Swedish Zeoliter är en grupp av kisel-aluminium-föreningar, med karaktäristiska kanalsystem inuti strukturen, som har fått stor användning inom många områden inom teknik och vetenskap. Zeoliternas viktigaste användningsområden är som torkmedel, som molekylsiktar, som jonbytare och som katalysatorer. Användningen av naturligt förekommande zeoliter är relativt liten jämfört med aA highly dealuminated form of zeolite FAU, ultra-stable zeolite Y (USY), was used as an adsorbent matrix in the purification of proteins. Undesired proteins were removed from a crude preparation by adsorption on USY, increasing the purity of an unadsorbed protein (horse radish peroxidase), or the protein of interest was adsorbed on the zeolite and subsequently eluted through changes in the solutio

Popular Abstract in Swedish Betydelsen av tryckinducerad syrgastransport hos övervattensväxter Min avhandling handlar om vikten av att syrgas transporteras till rötter och rhizom hos övervattensväxter som bladvass, kaveldun och säv. Syrgas behövs för cellandningen i alla växtdelar, och i synnerhet i rötterna då de växer fort och har hög ämnesomsättning. Övervattensväxter har rötterna i vattendränThis thesis examines factors regulating growth and distribution of emergent plants with focus on effects of oxygen supply to the roots. Greenhouse experiments revealed that P. australis acclimatizes to specific water depths with phenotypic plasticity. These phenotypic responses act to increase growth at specific water depths but may result in decreased tolerance to changed water depth. Oxygen nee

An ability to interact with plasminogen or plasmin could provide micro-organisms with a mechanism for invasion. Thus, group A, C and G streptococci secrete streptokinase which binds and activates plasminogen. Some streptococci also express surface structures which bind plasminogen without causing its activation. Plasminogen-binding surface proteins were extracted from one group C and one group G s

The aim of this study is to generate a quality-controlled sub-kilometer dataset of the fraction of absorbed photosynthetically active radiation (FAPAR) across Scandinavia from satellite. FAPAR is required for estimating the amount of PAR absorbed (APAR) by vegetation, which in turn allows for estimation of carbon uptake. In this study, FA-PAR was modeled from normalized difference vegetation index

Popular Abstract in Swedish Papillomvirus infekterar ett brett spektrum av ryggradsdjur, såsom människa, olika ap-arter, ko, älg, elefant, ren och bofink. Dessa virus kan ge så vitt skilda symptom som cancer och vårtor. De indelas efter vilken art de infekterar, och inom arterna efter skillnad i DNA-sekvens mellan olika isolat (typer). Hittills har 92 humana papillomvirus (HPV) typer beskrivits, oPapillomaviruses are a diverse, epitheliotropic group of viruses. Some genotypes are the main cause of anogenital cancer in humans; others can induce skin lesions. In order to investigate the occurrence of subclinical skin infections with human papillomavirus (HPV), a pair of degenerate primers (FAP59/64) was designed. Moist cotton-tipped swabs were used as a gentle method of sample collection. Di

The Swedish Pompeii Project started in 2000 as a research and fieldwork activity initiated by the Swedish Institute in Rome. The aim was to record and analyze an entire Pompeian city-block, Insula V 1. Since autumn 2011 a new branch of advanced digital archaeology, involving 3D reconstructions and documentation methods, was added to the project agenda. The insula was completely digitized using las