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We have previously shown that protein kinase Cepsilon (PKCepsilon) induces neurite outgrowth via its regulatory domain and independently of its kinase activity. This study aimed at identifying mechanisms regulating PKCepsilon-mediated neurite induction. We show an increased association of PKCepsilon to the cytoskeleton during neuronal differentiation. Furthermore, neurite induction by overexpressi
A novel and facile preparation method for spherical molecularly imprinted polymer (MIP) beads is presented. Two types of beads were synthesized and investigated: (i) silica-MIP composites were obtained by filling spherical, porous C-4-coated silica beads with print molecule and monomers followed by polymerisation; (ii) spherical molecularly imprinted polymer beads were acquired mirroring the silic
We report a flow-injection biosensor system with a capacitive transducer for assay and quality control of human immunoglobulin G (hIgG). The sensing platform is based on self-assembled monolayers (SAMs) of carboxylic acid terminated alkyl-thiols with covalently attached concanavalin A. The electrochemical characteristics of the sensor surface were assessed by cyclic voltammetry using a permeable r
The aggregating proteoglycans (aggrecan, versican, neurocan, and brevican) are important components of many extracellular matrices. Their N-terminal globular domain binds to hyaluronan, but the function of their C-terminal region containing a C-type lectin domain is less clear. We now report that a 90-kDa protein copurifies with recombinant lectin domains from aggrecan and versican, but not from t
This paper presents some improvements of the differential equation algorithm for transmission line protection. The improvements are: fault classification; new filtering; new algorithm for three-phase faults. Simulation results indicate a nominal operation time of 5-7 ms for three-phase faults and slightly higher for other fault types.
The binding of Helicobacter pylori to glycosphingolipids was examined by binding of 35S-labeled bacteria to glycosphingolipids on thin-layer chromatograms. In addition to previously reported binding specificities, a selective binding to a non-acid tetraglycosylceramide of human meconium was found. This H. pylori binding glycosphingolipid was isolated and, on the basis of mass spectrometry, proton
This study examined the role of P and type 1 fimbriae for neutrophil migration across Escherichia coli-infected uroepithelial cell layers in vitro and for neutrophil recruitment to the urinary tract in vivo. Recombinant E. coli K-12 strains differing in P or type 1 fimbrial expression were used to infect confluent epithelial layers on the underside of transwell inserts. Neutrophils were added to t
By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of prot
We tested the hypothesis that populations in small habitat fragments remaining in agricultural landscapes are maintained by repeated immigration, using three grassland butterflies (Aphantopus hyperantus, Coenonympha pamphilus and Maniola jurtina). Transect counts in 12 matched sets of semi-natural pastures, and linear habitat elements proximate and isolated from the pastures showed that population
In a Northern country such as Sweden, energy use in the home may be reduced by 20% through changes in behaviour. However, little is known about how households respond to policy instruments encouraging such change or to what degree this in turn may affect the workload of women and men in such communities. The current study presents findings from interviews with 30 households in Sweden that particip
n instrument suitable for measuring the total water content both as small liquid droplets and as vapour in the air, in water mist total flooding extinguishing tests, is presented. The instrument consists of a heater and a Vaisala humidity meter. The Vaisala humidity meter is capable of measuring relative humidity at temperatures up to 180°C. The instrument has a measuring range of 0-600g/m3, a sam
The COST/IWA benchmark simulation model has been available for seven years. Its primary purpose has been to create a platform for control strategy benchmarking of activated sludge processes. The fact that the benchmark has resulted in more than 100 publications, not only in Europe but also worldwide, demonstrates the interest in such a tool within the research community In this paper, an extension
Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single ex
Intensities of infrared absorption due to asymmetric stretching vibrations of interstitial oxygen atoms in Ge crystals enriched with O-16 and O-18 isotopes have been compared with oxygen concentrations determined by means of secondary ion mass spectrometry (SIMS). For Ge samples with oxygen content less than 510(17) cm(-3) a good correlation has been found between the values of oxygen concentratio
A previously presented robust and fast diagnostic NOx model was modified into a predictive model. This was done by using simple yet physically based models for fuel injection, ignition delay, premixed heat release rate and diffusion combustion heat release rate. The model can be used both for traditional high-temperature combustion and for high-EGR, low-temperature combustion. It was possible
Modern IC-engines produce more power per displaced volume and operate at higher combustion pressures and temperatures. In addition, engine structure materials such as aluminium and magnesium alloys, which are more sensitive to thermal loads, are more commonly used. These two parameters might lead to problems such as thermal fatigue, knock, high fuel consumption and emissions, performance loss, bor
