Filetype
The barley (Hordeum vulgare L.) mutants fch2 and clo-f2 comprise an allelic group of 14 Chl b-deficient lines. The genetic map position of fch2 corresponds to the physical map position of the gene encoding chlorophyllide a oxygenase. This enzyme converts chlorophyllide a to chlorophyllide b and it is essential for Chl b biosynthesis. The fch2 and clo-f2 barley lines were shown to be mutated in the
Lake sediments from the Magherus, Valley in the lowlands of northern Transylvania provide new evidence of paleoenvironmental development in Romania during the Late Glacial. The studied sediments were deposited as a result of the damming of a small river valley following a series of mass wasting events that occurred during the deglaciation period. A continuous sedimentary sequence belonging to the
Immunotherapy with intratumoral injection of adenoviral vectors expressing CD40L has yielded positive results in experimental and clinical bladder cancer. We therefore hypothesized that anti-CD40 antibody would be effective in this setting. Agonistic CD40 antibodies were developed as vaccine adjuvants but have later been used as treatment of advanced solid tumors and hematologic cancers. Systemic
Aims Biogeographical evidence suggests a strong link between climate and patterns of species diversity, and climate change is known to cause range shifts. However, there is little understanding of how shifts affect community composition and we lack empirical evidence of recent impacts of climate change on the diversity of vertebrates. Using a long-term comprehensive dataset on bird abundance, we e
Waist circumference (WC) and waist-to-hip ratio (WHR) are surrogate measures of central adiposity that are associated with adverse cardiovascular events, type 2 diabetes and cancer independent of body mass index (BMI). WC and WHR are highly heritable with multiple susceptibility loci identified to date. We assessed the association between SNPs and BMI-adjusted WC and WHR and unadjusted WC in up to
In the in vitro motility assay, actin filaments are propelled by surface-adsorbed myosin motors, or rather, myosin motor fragments such as heavy meromyosin (HMM). Recently, efforts have been made to develop actomyosin powered nanodevices on the basis of this assay but such developments are hampered by limited understanding of the HMM adsorption geometry. Therefore, we here investigate the HMM adso
Background:Merkel cell carcinoma (MCC) is an aggressive neuroendocrine tumour of the skin that has been associated with a new tumour virus, the MCC polyomavirus.Methods:To investigate whether MCC may have a shared aetiology with other cancers, we investigated the risk of second cancers after the diagnosis of MCC using the national cancer registries in Denmark, Norway and Sweden.Results:The overall
(PRELP)-P-hbd is a peptide corresponding to the N-terminal heparin binding domain of the matrix protein proline/arginine-rich end leucine-rich repeat protein (PRELP). (PRELP)-P-hbd inhibits osteoclastogenesis entering pre-fusion osteoclasts through a chondroitin sulfate- and annexin 2-dependent mechanism and reducing the nuclear factor-B transcription factor activity. In this work, we hypothesized
Protein affinity reagents (PARs), most commonly antibodies, are essential reagents for protein characterization in basic research, biotechnology, and diagnostics as well as the fastest growing class of therapeutics. Large numbers of PARs are available commercially; however, their quality is often uncertain. In addition, currently available PARs cover only a fraction of the human proteome, and thei
The subject of this thesis is the clinical use of the oesophageal electrode in electrocardiology. The three areas covered are oesophageal electro- cardiography, the biophysics of transoesophageal atrial stimulation (TAS) and the clinical utility of TAS. The literature is reviewed with respect to these areas. TAS was performed in 64 patients with documented supraventricular tachycardia (SVT) or a
Popular Abstract in Swedish Utveckling av blodbildande celler sker i benmärgens mikromiljö, som består av cytokiner och tillväxtfaktorer, stromaceller samt extracellulära matrixmolekyler. Extracellulära matrixproteiner och stromaceller i benmärgen utgör en strukturell miljö för hematopoietiska celler, men adhesiva cell-cell och cell-matrix interaktioner påverkar också direkt hematopoietiska cellerDevelopment of hematopoietic cells in bone marrow takes place in association with the hematopoietic microenvironment. Extracellular matrix (ECM) molecules and stromal cells in bone marrow provide a structural environment for hematopoietic cells, but they also directly influence proliferation, differentiation and migration of cells by signal transduction mediated by ligand binding to cell surface a
Popular Abstract in Swedish Atmosfäriskt nedfall är den största källan till miljögiftet PCB i sötvatten. I flertalet vattendrag, exempelvis Emån, fungererar också sediment som en källa. Ett pappersbruk i anslutning till Emån hade använt returpapper som innehöll PCB som råvara. PCB följde med utsläppen till ån och lagrades in i sedimenten. Miljögiftet läckte sedan ut långsamt till vattnet. Största Atmospheric deposition is one source of persistent pollutants such as polychlorinated biphenyls (PCB) in aquatic environments. Other point sources can also contribute to the PCB loadings in the water. In the Emån River, in southeast Sweden, contaminated sediment was a source of PCB, the largest deposits of it being found in a small lake connected with the river, Lake Järnsjön, the sediment of it c
Popular Abstract in Swedish Pyrimidiner är DNA-byggstenar som är essentiella för cellens överlevnad och delning. I mänskliga celler finns pyrimidiner att tillgå antingen genom återupptag eller genom biosyntes (nysyntes). Biosyntesen av pyrimidiner sker i flera steg och i ett av dem spelar enzymet Dihydroorotat dehydrogenas (DHODH) en avgörande roll genom att katalysera en oxidationsreaktion. När cIn the first part of the PhD work, small chemical entities (fragments) found to inhibit human dihydroorotate dehydrogenase (DHODH), were chemically optimized. As part of an ample program at Active Biotech to find new drugs against autoimmune diseases, expanded fragments were repeatedly designed, synthesized and evaluated in a human DHODH assay. Potent inhibitors were identified within three classe
Popular Abstract in Swedish Vi har studerat hur man kan använda immunsystemet för att behandla råttor med hjärntumör (maligna gliom). Eftersom råttor väldigt sällan utvecklar hjärntumörer, så måste vi injicera tumörceller i hjärnan själva. Om djuren inte får någon behandling dör de vanligen inom ca. 24 dagar. Får de däremot en injektion i buken eller under huden på benet med genmanipulerade tumörcMalignant glioma is, with rare exceptions in younger adults, an uncurable brain tumor, as very few survive more than 1-2 years after diagnosis. Despite advances in recent years, current treatments such as surgery, radiotherapy and chemotherapy only prolongs the life of the patients. Because of the poor prognosis, several additional therapies have been investigated and are under development, such a
Popular Abstract in Swedish Forskning om stamceller har fått mycket uppmärksamhet de senaste åren. Mycket av intresset avspeglar möjligheten att använda denna typ av celler som en terapi för att bota olika sjukdomar. En stam cell från hjärnan är en cell som har förmåga att dela sig symmetriskt, varvid två kopior bildas eller asymmetriskt, vilket ger upphov till en ny stam cell samt en progenitorceIn the present thesis, we have examined the in vivo properties of in vitro expanded human and rat neural stem-and progenitor cells after transplantation into the neonatal and adult rat brain. The survival and differentiation of the grafted cells were assessed using species-specific antisera, and pre-labelling with the reporter gene green fluorescent protein (GFP). Long-term growth factor-expanded
Popular Abstract in Swedish Inflammatoriska ledsjukdomar, innefattande bl.a. ledgångsreumatism, reumatoid artrit, och ledinflammation vid psoriasis, psoriasisartrit, är vanliga. I västvärlden lider cirka 1 procent av den vuxna befolkningen av reumatoid artrit (RA) medan psoriasisartrit (PsoA) är mindre vanlig. Ett kännetecken för dessa sjukdomar är att de medför en risk att ledernas vävnader brytsThe pathogenesis of inflammatory arthritides include synovial inflammation and joint tissue destruction. Tissue destruction has traditionally been regarded a consequence of inflammation, but recent observations indicate that the processes are not always closely linked and may proceed uncoupled. The ultimate target organs for the destructive process are articular cartilage and bone. This thesis f
