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This thesis focuses on the C-terminal G3 region of the large aggregating proteoglycans that are found in complex with hyaluronan in the extracellular matrix. The G3 region contains a C-type lectin domain that binds different extracellular matrix proteins playing an integral role in tissue organization during development and remodeling. Here we affinity purify the proteoglycan aggrecan via one of i
Purpose: To investigate the retinal structure and function during the progression of X-linked retinoschisis (XLRS) from childhood to adulthood. Methods: Ten patients clinically diagnosed with XLRS were investigated at 6-15 years of age (mean age 9 years) with a follow-up 8 to 14 years later (mean 12 years). The patients underwent regular ophthalmic examination as well as testing of best corrected
The Second International Conference on Particle Induced X-ray Emission (PIXE) and its analytical applications was held in Lund, Sweden, June 9-12, 1980. About a hundred papers were presented, including seven invited talks (PIXE and particle scattering, microbeam analysis (2), applications to aerosols (2) and biological samples (2)). The main impression left by the conference was that both the PIXE
Growth anomaly of trees in some regions was detected under current episode of rapid warming. Thisraises a dilemma for temperature reconstructions by using tree-ring width which is believed to be themost important proxy on inter-annual temperature reconstruction during the past millenniums. Here weemployed the tree-ring d13C to reconstruct temperature variations for exploring their potential oncapt
PURPOSEHuman papillomavirus type 16 (HPV16) infection is causing an increasing number of oropharyngeal cancers in the United States and Europe. The aim of our study was to investigate whether HPV antibodies are associated with head and neck cancer risk when measured in prediagnostic sera. METHODSWe identified 638 participants with incident head and neck cancers (patients; 180 oral cancers, 135 oro
Children with lesbian, gay, bisexual, trans* and/or queer (LGBTQ) parents have received heightened attention during the processes of policy making regarding adoption and donor insemination legislation in Sweden during the 1990s and early 2000s. Yet, very little academic knowledge exists about children and young people in LGBTQ families and their experiences in schools. We engage the idea of "cultu
Popular Abstract in Swedish Osteoporos (benskörhet) är en sjukdom som drabbar de äldre och leder till att skelettet urkalkas och därmed förlorar delar av sin styrka. Detta bidrager till olika typer av frakturer (brott av benet) där de vanligaste är handleds-, axel-, kot- och höftfrakturer. Andelen frakturer ökar i befolkningen, vilket både medför ökade kostnader för samhället och invaliditet för dThe aim of this thesis was to evaluate bone mass and bone size in girls and boys from age 12 to 16, with special attention to physical activity. We also wanted to retrospectively evaluate the effect of exercise for the years preceding age 12. The findings were as follows. Sixteen year old adolescents in a rural area had higher areal bone mineral density in the total body and the spine compared wit
Popular Abstract in Swedish Då reumatisk värk och artros är en växande folksjukdom har vi valt att studera proteiner som är involverade i dessa sjukdomar. Vi har arbetat med proteiner som finns i utrymmet utanför cellerna, dvs. den extracellulära matrisen. Cellerna formar sin egen närliggande miljö genom att utsöndra diverse proteiner. Dessa proteiner bildar sedan ett komplext nätverk med varandraThe cells of higher organisms exist in a complex protein network, the extracellular matrix (ECM), which has a wide variety of different components such as glycoproteins, proteoglycans and carbohydrates. As well as being the physical scaffold on which the cells are dependent for their positioning and mobility, the ECM exercises control over the cells, and changes in the matrix will affect the adhes
Aims: Climate change is known to drive both the reshuffling of whole assemblages and range shifts of individual species. Less is known about how local colonizations and extinctions of individual species contribute to changes at the community level. Our aim was to estimate the contribution of individual species to a change in community composition attributed to climate change and to relate these sp
The fate of phosphate in the environment is governed by reactions at particle surfaces. These adsorption and desorption reactions display biphasic kinetics involving an initial rapid reaction followed by a substantially slower one extending over long time periods. In this study we have investigated the molecular mechanisms of desorption kinetics of phosphate from ferrihydrite and goethite nanopart
Bacterial genomes typically consist of a single chromosome and, optionally, one or more plasmids. But whole-genome sequencing reveals about ten per-cent of them to be multipartite, with additional replicons which by size and indispensability are considered secondary chromosomes. This raises the questions of how their replication and partition is managed without compromising genome stability and of
Telomeres are protective cap structures at the ends of the linear eukaryotic chromosomes, which provide stability to the genome by shielding from degradation and chromosome fusions. The cap consists of telomere-specific proteins binding to the respective single- and double-stranded parts of the telomeric sequence. In addition to the nucleation of the chromatin structure the telomere-binding protei
The DNA ploidy level of 673 accessions belonging to 238 species of Hieracium have been analyzed by flow cytometry. 222 of the species were of Scandinavian origin as members of the exclusively apomictic H. sectt. Bifida, Hieracium, Oreadea, Tridentata and Vulgata. For the overwhelming majority of the species, the ploidy level (or chromosme number) has never been investigated before. Approximately 5
Objective: To develop an enzyme linked immunosorbent assay (ELISA) to quantify the levels of specific aggrecan fragments generated by aggrecanase-mediated cleavage at the (373)Glu-(374)Ala bond within the aggrecan interglobular domain. Methods: The ELISA employs a commercially available monoclonal antibody to capture aggrecan fragments containing keratan sulfate (KS). Aggrecan fragments generated
We present a new method for quantification of the coupling efficiency between amphiphilic oligonucleotides and suspended small unilamellar lipid vesicles (SUVs). The method employs a supported (phospho) lipid bilayer (SLB)-modified sensor template, which upon exposure to a mixture of SUVs and amphiphilic DNA reacts neither with free SUVs nor with DNA-modified SUVs, but with free DNA only. Using ca
Previous cytogenetic and comparative genomic hybridization (CGH) analyses have shown that the gain of chromosome arm 12p is frequent in pancreatic carcinomas. We investigated 15 pancreatic carcinoma cell lines using CGH, fluorescence in situ hybridization (FISH), and semiquantitative polymerase chain reaction (PCR) to characterize 12p amplifications in detail. The CGH analysis revealed gains of 12
