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A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor with
The importance of prioritising requirements is widely recognised. A number of different techniques for prioritising requirements have been proposed, some based on an ordinal scale, others on a ratio scale. Some measurement scales provide more information than others, i.e. the ratio scale is richer than the ordinal scale. This paper aims to investigate the differences between the scales used in pri
We here describe two proteomic datasets deposited in ProteomeXchange via PRIDE partner repository [1] with dataset identifiers PXD000484 (defined as "training") and PXD000485 (defined as "test") that have been used for the development of a tamoxifen outcome predictive signature [2]. Both datasets comprised 56 fresh frozen estrogen receptor (ER) positive primary breast tumor specimens derived from
Since the last decade, development efforts by academia and industry for automated driving functions have increased significantly. Also, the European research project AdaptIVe is looking into this topic. Due to the large operation spaces and various complex situations that are covered by these functions, efforts for evaluation increase also significantly. Within AdaptIVe, a comprehensive evaluation
Observational learning is a successful method for improving writing skills in various genres. We explore effects of a five lesson intervention series based on peer observation. Fifty-five Swedish 5th-grade students aged 10-12 years followed this intervention program. The language and reading comprehension and working memory capacity were tested. The students watched short film-clips with peers wor
OBJECTIVES: To evaluate health-related quality of life (HRQoL) and its determinants in a systemic sclerosis (SSc) multinational inception cohort. We performed a meta-analysis of data from individual countries, and compared the meta-analysis to individual country results by pooling data from each of the countries.METHODS: SSc patients within 2 years of disease onset were recruited from 5 countries
Polyamines are cationic molecules synthesized via a highly regulated pathway, obtained from the diet or produced by the gut microbiota. They are involved in general molecular and cellular phenomena that play a role also in vascular disease. Bicuspid aortic valve (BAV) is a congenital malformation associated to a greater risk of thoracic ascending aorta (TAA) aneurysm, whose pathogenesis is not yet
In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day op
Predicted impacts of climate change on crops—including yield declines and loss of conservation lands—could be mitigated by exploiting existing diversity within crops. Here we examine this possibility for wine grapes. Across 1,100 planted varieties, wine grapes possess tremendous diversity in traits that affect responses to climate, such as phenology and drought tolerance. Yet little of this divers
Boreal trees rely on their ectomycorrhizal fungal symbionts to acquire growth-limiting nutrients, such as nitrogen (N), which mainly occurs as proteins complexed in soil organic matter (SOM). The mechanisms for liberating this N are unclear as ectomycorrhizal fungi have lost many genes encoding lignocellulose-degrading enzymes present in their saprotrophic ancestors. We hypothesized that hydroxyl
OBJECTIVE: To describe the epidemiology of illness at the Rio 2016 Summer Paralympic Games.METHODS: A total of 3657 athletes from 78 countries, representing 83.5% of all athletes at the Games, were monitored on the web-based injury and illness surveillance system (WEB-IISS) over 51 198 athlete days during the Rio 2016 Summer Paralympic Games. Illness data were obtained daily from teams with their
Brain structure and function are tightly correlated across all animals. While these relations are ultimately manifestations of differently wired neurons, many changes in neural circuit architecture lead to larger-scale alterations visible already at the level of brain regions. Locating such differences has served as a beacon for identifying brain areas that are strongly associated with the ecologi
Background: Laparoscopic distal pancreatectomy (LDP) appears to be safe and effective as open distal pancreatectomy (ODP) for benign or borderline malignant lesion. However, studies comparing LDP with ODP in elderly patients are limited. The purpose of this study is to compare the clinical outcomes of these two several approaches in elderly patients. Methods: A retrospective analysis was carried o
OBJECTIVE(S):: We explored incidence, predictors, and long-term prognosis of hospital admissions attributed to reflex syncope and orthostatic hypotension. METHODS:: We analyzed a cohort of 32?628 individuals (68.2% men; age, 45.6?±?7.4 years) without prevalent cardiovascular disease over a follow-up period of 26.6?±?7.5 years. RESULTS:: One thousand and fourteen persons (3.1%, 1.2 per 1000 person-
This paper discusses the design and analysis of a reflector antenna system combining two doubly-curved Circular Polarization Selective Surfaces to provide a functionality equivalent to that of a dual gridded reflector but in circular polarization. The key feature of this design is to use a non-resonant CPSS design to avoid the bandwidth limitation of most CPSS designs previously discussed in the l
The activation of the complement system is a key initiating step in the protective innate immune-inflammatory response against injury, although it may also cause harm if left unchecked. The structurally related soluble complement inhibitors C4b-binding protein (C4BP) and factor H (FH) exert a tight regulation of the classical/lectin and alternative pathways of complement activation, respectively,
Theoretical investigations of the structure and function of the blue copper proteins are described. We have studied the optimum vacuum geometry of oxidised and reduced copper sites, the relative stability of trigonal and tetragonal Cu(II) structures, the relation between the structure and electronic spectra, the reorganisation energy, and reduction potentials. Our calculations give no support to t
Scope: Multifunctional diet (MFD), a diet based on multiple functional concepts and ingredients with anti-inflammatory activity, was previously shown to improve different cardiometabolic risk-associated markers in healthy subjects. Here, we assessed the impact of MFD on plasma metabolome and explored associations of the differential metabolites with clinical parameters, searching for metabolic det
This chapter focuses on measuring spatial pattern in floodplains and reviews 108 publications from 1934-2013 to determine trends, dominant paradigms, and approaches to measuring spatial pattern in floodplains. The development of new technologies, especially those associated with remotely sensed data capture, increases the ability to quantitatively measure the spatial complexity of floodplain surfa
Ni-YSZ (nickel - yttria stabilized zirconia) is a material widely used for electrodes and supports in solid oxide electrochemical cells. The mechanical and electrochemical performance of these layers, and thus the whole cell, depends on their microstructure. During the initial operation of a cell, NiO is reduced to Ni. When this process is conducted under external load, like also present in a stac
