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Integrin dependent adhesion and dynamic modulations of the actin network are prerequisites for normal cell locomotion. To investigate whether the actin microfilamentous system does play a role in regulation of β2-integrin-induced signalling, we pretreated granulocytes with staurosporine, a well-known protein kinase inhibitor that has also been shown to disrupt the cytoskeleton of intact cells. Pre
Since the seriousness and urgency of climate change has surfaced during the last decade, researchers, experts, and even the pope have argued that dealing with it is a moral issue. However, emissions keep rising and it seems as if few people in the north are ready and willing to make any substantial lifestyle changes to minimize the potentially catastrophic consequences, mainly to the south. Intern
Ions differ in their ability to salt out proteins from solution as expressed in the lyotropic or Hofmeister series of cations and anions. Since its first formulation in 1888, this series has been invoked in a plethora of effects, going beyond the original salting out/salting in idea to include enzyme activities and the crystallization of proteins, as well as to processes not involving proteins lik
Patients with intracranial tumours have an increased risk of venous thromboembolism, particularly during the first month after neurosurgery. A proposed explanation for this increased risk, are procoagulant tumour-derived substances, such as tissue factor, usually measured in peripheral blood. The aim of the present study is to investigate whether a rotational thromboelastometry (ROTEM) can measure
Collaboration between modellers and experimentalists is essential in ecological research, however, different obstacles linking both camps often hinder scientific progress. In this commentary, we discuss several issues of the current state of affairs in this research loop. Backed by an online survey amongst fellow ecologists, modellers and experimentalists alike, we identify two major areas that ne
An experimental investigation of the tip flow characteristics between a gas turbine blade tip and the shroud was conducted by a pressure-test system and a particle image velocimetry (PIV) system. A three-times scaled profile of the GE-E3 blade with five film cooling holes was used as specimen. The effects on flow characteristics by the rim width and the groove depth of the squealer tip were reveal
Statistics on African economic growth are widely known to be inaccurate, but the extent and nature of these inaccuracies and their implications for the users of the data have not been rigorously assessed. This article investigates measurement issues of economic growth in post-colonial Tanzania. It is shown here that conclusions on Tanzania's development performance are conditioned by selection of
Few, if any, corporate governance related issues have been given as much research interest as ownership dispersion and ownership concentration of listed corporations: ‘Why is that some countries, such as the US and UK, has dispersed ownership of corporations listed there, while others, such as France and Germany, has concentrated ownership of corporations listed there?’. This is a question that ha
In spite of the fact that hatchling size and energy reserves in birds are affected by egg size, many studies have failed to find an effect of egg size on offspring fitness. One possibility is that this is because they have been performed in areas with high food availability and that effects of egg size on offspring fitness are most apparent in areas of low food availability. To investigate this, e
We have found that the dissolution of cellulose in the binary mixed solvent tetrabutylammonium acetate/dimethyl sulfoxide follows a previously overlooked near-stoichiometric relationship such that one dissolved acetate ion is able to dissolve an amount of cellulose corresponding to about one glucose residue. The structure and dynamics of the resulting cellulose solutions were investigated using sm
The Bacillus subtilis hemAXCDBL operon encodes enzymes for the synthesis of 5-aminolaevulinic acid via the C5 pathway (hemA and hemL) and uroporphyrinogen III (hemB, hemC and hemD). B. subtilis HemA protein (molecular mass 50 kDa) was overexpressed in hemA mutants of both Escherichia coli and B. subtilis. A mutant B. subtilis HemA protein with a Cys to Tyr change at position 105 was also overexpre
Early agriculture can be detected in palaeovegetation records, but quantification of the relative importance of climate and land use in influencing regional vegetation composition since the onset of agriculture is a topic that is rarely addressed. We present a novel approach that combines pollen-based REVEALS estimates of plant cover with climate, anthropogenic land-cover and dynamic vegetation mo
Aim: The treatment of peritoneal surface malignancies ranges from palliative care to full cytoreductive surgery (CRS) and heated intraperitoneal chemotherapy, HIPEC. Ongoing monitoring of patient recruitment and volume is usually carried out through dedicated registries. With multiple registries available worldwide, we sought to investigate the nature, extent and value of existing worldwide CRS an
Large eddy simulations (LES) of a stirred reactor with a liquid volume of 0.64 m3 were performed. The tank was stirred by either two standard Rushton impellers or two Scaba 6SRGT impellers. The LES can provide details of the flow field that cannot be obtained with so‐called Reynolds‐averaged equations and the corresponding models. The unsteady spatially filtered incompressible Navier‐Stokes equati
The introduction of spraying procedures to fabricate layer-by-layer (LbL) films has brought new possibilities for the control of molecular architectures and for making the LbL technique compliant with industrial processes. In this study we show that significantly distinct architectures are produced for dipping and spray-LbL films of the same components, which included DODAB/DPPG vesicles. The film
