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Protein identification by in-gel digestion, high-performance liquid chromatography, and mass spectrometry: peptide analysis by complementary ionization techniques

A biologically active protein fraction was isolated from rabbit intestine, purified by one-dimensional SDS-PAGE and stained with Coomassie Brilliant Blue. A predominant band of approximately 110-130 kDa was excised and digested in-gel with trypsin. The resulting peptides were extracted then separated by microbore reversed-phase high-performance liquid chromatography (HPLC). Mass spectrometric data

Quantum Information and Computing for Beginners : Basics of Qubit Transformations and Quantum Algorithms

Quantum information and quantum computing are based on the concept and manipulation of qubits. In this pedagogical Tutorial-written with the aim of assisting self-motivated undergraduate mathematics, physics, and engineering students-transformation of these qubits, especially the Hadamard transformation (H) and the phase shifter transformation (φ), is illustrated. Two-qubit gates and their applica

Management of hydrogen sulfide in anaerobic digestion of enzyme pretreated marine macro-algae

Enzymatic pretreatment of algae by means of cellulose degrading enzyme was evaluated through lab-scale and pilot-scale experiments. The degradation efficiency of the enzyme depended on the initial physical quality of the algae. Lab-scale batch anaerobic digestion experiments showed comparatively low methane potential for the pretreated algae at both mesophilic and thermophilic temperatures. Howeve

Mucosal glucagon-like peptide-1 and gastric inhibitory polypeptide cell numbers in the super-obese human foregut after gastric bypass.

Super-obesity, a body mass index>50 kg/m(2), is difficult to treat. Many studies have focused on the anatomic changes of the intestines; the physiologic background is not clearly identified. It is established that Roux-en-Y gastric bypass (RYGB) augments secretion of glucagon-like peptide-1 (GLP-1), peptide tyrosine tyrosine (PYY), and insulin, but other aspects of gut hormone cell function in the

Inhibitory effect of barusiban and atosiban on oxytocin-induced contractions of myometrium from preterm and term pregnant women

BACKGROUND: A synthetic oxytocin analogue, barusiban, was shown to potently inhibit oxytocin-induced activity of myometrium from term pregnant women. The responsiveness to vasopressin was not influenced by the compound. OBJECTIVE: To test the effect of barusiban and a reference compound, atosiban, on oxytocin-induced activity of myometrium from women at preterm pregnancy in comparison to myometriu

One week sweating simulation test with a thermal foot model

This study aimed to find out how the insulation of the footwear changes over one week of use. A thermal foot model with 8 measuring zones and 5 “sweat glands” was used for tests. A thin sock was donned on the model to allow better water distribution all over the foot surface. Four boots were tested over a period of 5 days. Each day was divided into 9 hour measurement period and 15 hour conditionin

Avoiding procrastination and the planning fallacy : Implementing study-plans as a strategy to increase student achievement

Within a course in academic writing, first-term students of English currently construct individualized study-plans detailing both what they should do during the term and when they should do it. Students calculate the average workload for all their courses, and include in their study-plans all the hours they should spend both in and outside the classroom on each course, totalling in 40-hour working

Strain fields and mechanical response of a high to medium fissured bentonite clay

This article presents the developments of an ongoing research aimed at modelling the influence of fissuring on the behaviour of clays. In particular, it recalls the main results of an extensive laboratory investigation on a fissured bentonite clay from the south of Italy and presents the data of a new investigation on the evolution with shearing of the strain fields developing within the clay, res

Laser tumor thermotherapy: Is there a clinically relevant effect on the immune system

Laser thermotherapy is interesting from an immunological point of view since it can reduce tumor volume without causing immunosuppression at the same time as it may induce and/or enhance tumor immunity. In a rat liver tumor model, we have demonstrated that laser thermotherapy 1) is superior to surgical resection, 2) gives a strong rejection immunity associated with an immune cellular response of t