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The M17 leucine aminopeptidase of the malaria parasite Plasmodium falciparum : importance of active site metal ions in the binding of substrates and inhibitors

The M17 leucine aminopeptidase of the intraerythrocytic stages of the malaria parasite Plasmodium falciparum (PfLAP) plays a role in releasing amino acids from host hemoglobin that are used for parasite protein synthesis, growth, and development. This enzyme represents a target at which new antimalarials could be designed since metalloaminopeptidase inhibitors prevent the growth of the parasites i

Crystal structure of β-glucosidase 1A from Thermotoga neapolitana and comparison of active site mutants for hydrolysis of flavonoid glucosides

The β-glucosidase TnBgl1A catalyses hydrolysis of O-linked terminal β-glycosidic bonds at the nonreducing end of glycosides/oligosaccharides. Enzymes with this specificity have potential in lignocellulose conversion (degrading cellobiose to glucose) and conversion of bioactive flavonoids (modification of glycosylation results in modulation of bioavailability). Previous work has shown TnBgl1A to hy

Siliceous Raw Material Exploitation at Hort de la Boquera Site (Margalef de Monsant, Tarragona, España) : First Results from La-Icp-Ms Analysis

Research on the sourcing of siliceous raw materials in Prehistory has taken a step forward with the addition of geochemical techniques and elemental analysis to the archaeopetrological method. Counter to the macroscopic techniques, which retain an elevated subjective percentage in their results, geochemical techniques and especially LA-ICP-MS analysis generate objective, quantifiable and usable da