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Protein-binding RNA aptamers affect molecular interactions distantly from their binding sites.
Nucleic acid aptamer selection is a powerful strategy for the development of regulatory agents for molecular intervention. Accordingly, aptamers have proven their diligence in the intervention with serine protease activities, which play important roles in physiology and pathophysiology. Nonetheless, there are only a few studies on the molecular basis underlying aptamer-protease interactions and th
Screening the molecular surface of human anticoagulant protein C: a search for interaction sites
Protein C (PC), a 62 kDa multi-modular zymogen, is activated to an anticoagulant serine protease (activated PC or APC) by thrombin bound to thrombomodulin on the surface of endothelial cells. PC/APC interacts with many proteins and the characterisation of these interactions is not trivial. However, molecular modelling methods help to study these complex biological processes and provide basis for r
Locating the Hans Tausen drill site
Identification of Noncollagenous Sites Encoding Specific Interactions and Quaternary Assembly of alpha 3 alpha 4 alpha 5(IV) Collagen IMPLICATIONS FOR ALPORT GENE THERAPY
Multiple interferon regulatory factor and NF-κB sites cooperate in mediating cell-type- and maturation-specific activation of the human CD83 promoter in dendritic cells
CD83 is one of the best-known surface markers for fully mature dendritic cells (mature DCs), and its cell-type- and maturation-specific regulation makes the CD83 promoter an interesting tool for the genetic modulation of DCs. To determine the mechanisms regulating this DC- and maturation-specific CD83 expression, chromatin immunoprecipitation (ChIP)-on-chip microarray, biocomputational, reporter,
Pentadecamer-binding proteins : Definition of two independent protein-binding sites needed for functional activity
Triple A-Site Cation Mixing in 2D Perovskite-Inspired Antimony Halide Absorbers for Efficient Indoor Photovoltaics
Antimony-based perovskite-inspired materials (PIMs) are solution-processable halide absorbers with interesting optoelectronic properties, low toxicity, and good intrinsic stability. Their bandgaps around 2 eV make them particularly suited for indoor photovoltaics (IPVs). Yet, so far only the fully inorganic Cs3Sb2ClxI9−x composition has been employed as a light-harvesting layer in IPVs. Herein, th
Autumn migratory orientation and route choice in early and late dunlins Calidris alpina captured at a stopover site in Alaska
Institutional Barriers to On-Site Alternative Water Systems : A Conceptual Framework and Systematic Analysis of the Literature
‘A Balancing Act’: How Young Consumers Experience Brands’ Communicative Adjustments on Social Networking Sites
Small Scale Reformers for on-site Hydrogen Supply
The adsorption site of methoxy and ethoxy on Cu(100) as determined by vibrational spectroscopy and first principle calculations
A European aerosol phenomenology - 6 : Scattering properties of atmospheric aerosol particles from 28 ACTRIS sites
This paper presents the light-scattering properties of atmospheric aerosol particles measured over the past decade at 28 ACTRIS observatories, which are located mainly in Europe. The data include particle light scattering (σsp) and hemispheric backscattering (σbsp) coefficients, scattering Ångström exponent (SAE), backscatter fraction (BF) and asymmetry parameter (g). An increasing gradient of σsp
Site Characteristics Mediate the Relationship Between Forest Productivity and Satellite Measured Solar Induced Fluorescence
Solar-Induced Chlorophyll Fluorescence (SIF) can provide key information about the state of photosynthesis and offers the prospect of defining remote sensing-based estimation of Gross Primary Production (GPP). There is strong theoretical support for the link between SIF and GPP and this relationship has been empirically demonstrated using ground-based, airborne, and satellite-based SIF observation
Similar Active Sites and Mechanisms Do Not Lead to Cross-Promiscuity in Organophosphate Hydrolysis : Implications for Biotherapeutic Engineering
Organophosphate hydrolases are proficient catalysts of the breakdown of neurotoxic organophosphates and have great potential as both biotherapeutics for treating acute organophosphate toxicity and as bioremediation agents. However, proficient organophosphatases such as serum paraoxonase 1 (PON1) and the organophosphate-hydrolyzing lactonase SsoPox are unable to hydrolyze bulkyorganophosphates with
The growing season greenhouse gas balance of a continental tundra site in the Indigirka lowlands, NE Siberia
Crystal structure and biophysical properties of Bacillus subtilis BdbD: An oxidizing thiol:disulfide oxidoreductase containing a novel metal site
BdbD is a thiol: disulfide oxidoreductase (TDOR) from Bacillus subtilis that functions to introduce disulfide bonds in substrate proteins/peptides on the outside of the cytoplasmic membrane and, as such, plays a key role in disulfide bond management. Here we demonstrate that the protein is membrane-associated in B. subtilis and present the crystal structure of the soluble part of the protein lacki